TRAINING PROGRAM

Sand Fly Recognition and Identification

1.      Sand Fly Classification

        a.      General

        b.      Old World

        c.      New World

2.      Distinguishing sand flies from other flying insects.

3.      Distinguishing sand flies from other Psychodidae

4.      Identifying sand flies to species

        a.      Males distinguishing characteristics

        b.      Female distinguishing characteristics

        c.      Use of dichotomous keys

5.      Preparing Specimens for identification

6.      Mounting specimens for identification

7.      Establishing Reference Collections

 

Sand Fly Dissection and Examination for Leishmania

1.      Collection, preservation and preparation of fresh specimens

        a.      Live capture

        b.      Anesthetization/immobilization

        c.      Cryo preservation

2.      Dissection

        a.      Purpose

        b.      Materials

        c.      Procedure

3.      Examination

 

Sand Fly Dissection for Salivary Glands

1.      Collection, preservation and preparation of fresh specimens

2.      Dissection

        a.      Purpose

        b.      Materials

        c.      Procedure

3.      Preservation of salivary glands